TP53 Knockout cell line (RKO)

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location： Home > Products > KO Cell Lines > TP53 Knockout cell line (RKO)

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TP53 Knockout cell line (RKO) Order

TP53 Knockout cell line (RKO)
Catalog#
YKO-H1428
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TP53 Knockout cell line (RKO)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TP53 Knockout cell line (RKO)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Gene Name
TP53
Gene ID
7157
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 5 (based on transcript TP53-201)
Validation: PCR+Sanger Sequencing
TP53 Knockout cell line (RKO) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:RKO		 STR Info (Cell bank)
Cell Line:p53R [JHU-56]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11131113
D13S317 811811
D7S820 810810
D16S539 13131213
VWA 16221622
TH01 610610
AMEL XXXX
TPOX 11111111
CSF1PO 810810
D12S391 151920.0
FGA 202123.0
D2S1338 1616
D21S11 272930.0
D18S51 1112
D8S1179 91314.0
D3S1358 1619
D6S1043 1419
PENTAE 1113
D19S433 1414
PENTAD 1011
D1S1656 131417.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TP53 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the RKO cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TP53 gene knockout TP53 Knockout cell line (RKO) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TP53 Knockout cell line (RKO)
TP53 Knockout cell line (RKO)
Catalog#
YKO-H1428
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • TP53 Knockout cell line (Hep G2)

    TP53 Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: TP53
    Gene ID: 7157
    Size: 1*10^6
    Catalog#: YKO-HT1420
    YKO-HT1420(Hep G2)
  • TP53 Knockout cell line (THP-1)

    TP53 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: TP53
    Gene ID: 7157
    Size: 1*10^6
    Catalog#: YKO-HT0116
    YKO-HT0116(THP-1)
  • TP53 Knockout cell line(A549)

    TP53 Knockout cell line(A549)
      inquiry   More details

    Organism: Human
    Gene Name: TP53
    Gene ID: 7157
    Size: 1*10^6
    Catalog#: YKO-H563
    YKO-H563(A549)

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EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • TP53 Knockout cell line (Hep G2)

    TP53 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: TP53
    Gene ID: 7157
    Size: 1*10^6
    Catalog#: YKO-HT1420
      inquiry   More details
  • TP53 Knockout cell line (THP-1)

    TP53 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: TP53
    Gene ID: 7157
    Size: 1*10^6
    Catalog#: YKO-HT0116
      inquiry   More details
  • TP53 Knockout cell line(A549)

    TP53 Knockout cell line(A549)

    Organism: Human
    Gene Name: TP53
    Gene ID: 7157
    Size: 1*10^6
    Catalog#: YKO-H563
      inquiry   More details

TP53 Knockout cell line (RKO) Order

TP53 Knockout cell line (RKO)
Catalog#
YKO-H1428
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TP53 Knockout cell line (RKO)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TP53 Knockout cell line (RKO)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Gene Name
TP53
Gene ID
7157
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 5 (based on transcript TP53-201)
Validation: PCR+Sanger Sequencing
TP53 Knockout cell line (RKO) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:RKO		 STR Info (Cell bank)
Cell Line:p53R [JHU-56]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11131113
D13S317 811811
D7S820 810810
D16S539 13131213
VWA 16221622
TH01 610610
AMEL XXXX
TPOX 11111111
CSF1PO 810810
D12S391 151920.0
FGA 202123.0
D2S1338 1616
D21S11 272930.0
D18S51 1112
D8S1179 91314.0
D3S1358 1619
D6S1043 1419
PENTAE 1113
D19S433 1414
PENTAD 1011
D1S1656 131417.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TP53 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the RKO cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TP53 gene knockout TP53 Knockout cell line (RKO) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TP53 Knockout cell line (RKO)
TP53 Knockout cell line (RKO)
Catalog#
YKO-H1428
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • TP53 Knockout cell line (Hep G2)

    TP53 Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: TP53
    Gene ID: 7157
    Size: 1*10^6
    Catalog#: YKO-HT1420
    YKO-HT1420(Hep G2)
  • TP53 Knockout cell line (THP-1)

    TP53 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: TP53
    Gene ID: 7157
    Size: 1*10^6
    Catalog#: YKO-HT0116
    YKO-HT0116(THP-1)
  • TP53 Knockout cell line(A549)

    TP53 Knockout cell line(A549)
      inquiry   More details

    Organism: Human
    Gene Name: TP53
    Gene ID: 7157
    Size: 1*10^6
    Catalog#: YKO-H563
    YKO-H563(A549)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • TP53 Knockout cell line (Hep G2)

    TP53 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: TP53
    Gene ID: 7157
    Size: 1*10^6
    Catalog#: YKO-HT1420
      inquiry   More details
  • TP53 Knockout cell line (THP-1)

    TP53 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: TP53
    Gene ID: 7157
    Size: 1*10^6
    Catalog#: YKO-HT0116
      inquiry   More details
  • TP53 Knockout cell line(A549)

    TP53 Knockout cell line(A549)

    Organism: Human
    Gene Name: TP53
    Gene ID: 7157
    Size: 1*10^6
    Catalog#: YKO-H563
      inquiry   More details

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